Targeted and Repetitive Chromosomal Integration Enables High-Level Heterologous Gene Expression in Lactobacillus casei
نویسندگان
چکیده
منابع مشابه
Development of an Escherichia coli-Lactobacillus casei shuttle vector for heterologous protein expression in Lactobacillus casei.
There is an increasing interest to develop various lactic acid bacteria (LAB) species as mucosal delivery vehicles, for which the development of a variety of cloning and expression systems for these bacteria is of primary importance. This study reports the complete nucleotide sequence of the cryptic plasmid pRCEID7.6 derived from the chicken probiotic LAB strain Lactobacillus casei TISTR1341. S...
متن کاملChromosomal Insertions in the upp Gene of Lactobacillus Casei
1 Useful for the Expression of Vaccines 2 Bai-fen Song, Long-zhu Ju, Yi-jing Li, Li-jie Tang 3 aDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast 4 Agricultural University, 59 Mucai Street, Harbin, Heilongjiang Province, 150030, China 5 bCollege of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing, 6 Heilongjiang Province, 16331...
متن کاملHeterologous Expression of Mannanase and Developing a New Reporter Gene System in Lactobacillus casei and Escherichia coli
Reporter gene systems are useful for studying bacterial molecular biology, including the regulation of gene expression and the histochemical analysis of protein products. Here, two genes, β-1,4-mannanase (manB) from Bacillus pumilus and β-glucuronidase (gusA) from Escherichia coli K12, were cloned into the expression vector pELX1. The expression patterns of these reporter genes in Lactobacillus...
متن کاملHigh efficiency electrotransformation of Lactobacillus casei.
We investigated whether protocols allowing high efficiency electrotransformation of other lactic acid bacteria were applicable to five strains of Lactobacillus casei (12A, 32G, A2-362, ATCC 334 and BL23). Addition of 1% glycine or 0.9 M NaCl during cell growth, limitation of the growth of the cell cultures to OD600 0.6-0.8, pre-electroporation treatment of cells with water or with a lithium ace...
متن کاملCell surface characteristics of Lactobacillus casei subsp. casei, Lactobacillus paracasei subsp. paracasei, and Lactobacillus rhamnosus strains.
Hydrophilic and electrostatic cell surface properties of eight Lactobacillus strains were characterized by using the microbial adhesion to solvents method and microelectrophoresis, respectively. All strains appeared relatively hydrophilic. The strong microbial adhesion to chloroform, an acidic solvent, in comparison with microbial adhesion to hexadecane, an apolar n-alkane, demonstrated the par...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2019
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.00033-19